Size exclusion chromatography

Regina Valluzzi

Arlington, United States

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pigment ink and art marker on acid free paper, roughly 5 by 8 inches.
SOLD as a framed print

This is a pretty straightforward drawing of the principles in size exclusion separations and chromatography. Little porous beads are packed together, and a solution of molecules passes through the packed bed of beads. The large molecules are too big for the pores of the beads and they quickly pass straigth through the packed bed. Smaller molecules can explore more of the bead porosity and take a longer path – and longer time – through the bed. The underlying mechanism is different than, for example, paper chromatography, which you can try at home.

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